Reporter
Part:BBa_K1100018
Designed by: Chenxi He, Jiadong Ni Group: iGEM13_Fudan (2013-09-13)
R1051 with insulator and sfGFP
We fused the constitutive promoters to a sfGFP reporter to test their stochastic dynamics by means of measuring fluorescence intensity of sfGFP using flow cytometry. In the meantime, we inserted the 16nt Csy4 insulator between the promoter and the reporter to stardardize the expression of the reporter downstream by eliminating the influence from 5'UTR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
abs | B0034 |
biology | |
direction | Forward |
emission | 510nm |
excitation | 485nm |
negative_regulators | 1 |
positive_regulators | |
protein | sfGFP |